Call us
08045816757
Diluted patient serum is added to wells coated with purified dsDNA antigen. Specific antibody, if present,binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind tothe antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added.The plate is incubated to allow the oxidation of the substrate by the enzyme. The intensity of the colorgenerated is proportional to the amount of specific antibody in the sample.