Calbiotech Free Beta hCG Elisa Kit

Product Description

PRINCIPLE OF THE TEST
The Calbiotech free β-hCG kit is a solid phase sandwich assay method based on streptavidin-biotin principle.Standards, samples, and the biotinylated anti-free β-hCG antibody reagent are added into wells coated withStreptavidin. Free β-hCG in the samples binds to the biotinylated antibody. Simultaneously, the biotinylatedantibody binds to the Streptavidin coated plate. Unbound protein and excess biotin conjugated antibody arewashed off by wash buffer. Upon the addition of Peroxidase (HRP) conjugated anti-free β-hCG antibodyreagent, a sandwich complex is formed, where the free β-hCG being in between the two highly specificantibodies, labeled with biotin and HRP. Unbound protein and excess enzyme conjugated antibody reagent iswashed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directlyproportional to the concentration of free β-hCG in the samples. A standard curve is prepared relating colorintensity to the concentration of free β-hCG.