Calbiotech HE4 Elisa Kit.

Product Description

PRINCIPLE OF THE TEST
The HE4 kit is a solid phase sandwich assay method based on streptavidin-biotin principle.Standards, samples, and the biotinylated anti-HE4 antibody reagent are added into wells coatedwith Streptavidin. HE4 in the samples binds to the biotinylated antibody. Simultaneously, thebiotinylated antibody binds to the Streptavidin coated plate. Unbound protein and excess biotinconjugated antibody are washed off by wash buffer. Upon the addition of Peroxidase (HRP)conjugated anti- HE4 antibody reagent, a sandwich complex is formed, where the HE4 being inbetween the two highly specific antibodies, labeled with biotin and HRP. Unbound protein andexcess enzyme conjugated antibody reagent is washed off by wash buffer. Upon the addition of thesubstrate, the intensity of color developed is directly proportional to the concentration of HE4 in thesamples. A standard curve is prepared relating color intensity to the concentration of HE4.