Calbiotech LH Elisa Kit

Product Description

PRINCIPLE OF THE TEST
The LH ELISA kit is a solid phase assay using streptavidin/biotin method. The samples and Anti-LH/AntiBiotin conjugate are added to the wells coated with Streptavidin. LH in the patient™s serum forms asandwich between specific antibodies labeled with biotin and HRP. Unbound protein and HRP conjugateare washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional tothe concentration of LH in the samples. A standard curve is prepared relating color intensity to theconcentration of the LH.

SUMMARY AND EXPLANATION
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in responseto luteinizing hormone-releasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH,also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight ofapproximately 30,000 Dalton. It is composed of two noncovalently associated dissimilar amino acid chains,alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH),follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG). LH stimulates ovulation andovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LHis elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitarytumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency andectopic steroid production.

STORAGE AND STABILITY1. Store the kit at 2 - 8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.