Calbiotech RNP/ Sm IgG Elisa Kit.

Product Description

SUMMARY AND EXPLANATION
Systemic autoimmune disease is characterized by the presence of circulating auto-antibodiesdirected to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonlyreferred to as Lupus is the best known of these diseases. Other possible connective tissuediseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, andpolymyositis/dermatomyositis. The majority can be diagnosed by clinical presentation and theirantibody profiles to the various antigens involved, which include dsDNA, Sm, RNP, Ro, La Scl70, Jo1 and Histones. Therefore, immunoassays for autoantibodies are useful for diagnostic andprognostic evaluations of autoimmune disease. RNP Abs target U1 RNA 70kD, A or C proteinsand are present in most MCTD patients and in about 15% of SLE patients. The Sm or Smith Abstarget Bï/B or D proteins and are present in approximately 30% of patients with SLE . Sm is avery specific marker for SLE. Sm antibodies are very rare in other autoimmune diseases andnormals.

PRINCIPLE OF THE TEST
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, ifpresent, binds to the antigen. All unbound materials are washed away and the enzymeconjugate is added to bind to the antibody-antigen complex, if present. Excess enzymeconjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis ofthe substrate by the enzyme. The intensity of the color generated is proportional to the amountof IgG specific antibody in the sample.