Calbiotech TSH Elisa Kit

Product Description

PRINCIPLE OF THE TEST
The CBI TSH is a solid phase sandwich ELISA method. The samples and anti-TSHHRP/Biotin conjugate are added to the wells coated with Streptavidin. TSH in thepatient™s sample forms a sandwich between two specific antibodies to TSH. Unboundprotein and HRP conjugate are washed off by wash buffer. Upon the addition of thesubstrate, the intensity of color is proportional to the concentration of TSH in thesamples. A standard curve is prepared relating color intensity to the concentration ofthe TSH.

SUMMARY AND EXPLANATION
Thyroid Stimulating Hormone (TSH) is a glycoprotein hormone secreted by the pituitarygland and regulates the synthesis/ release of T3 and T4 by thyroid gland. TSH has twosubunits, namely alpha and beta. The alpha subunit is similar to the alpha subunitfound in LH, FSH and hCG glycoprotein hormones. However, the beta subunit isspecific and differs from hormone to hormone. The serum TSH measurement is one ofthe most important tools in the diagnosis of thyroid disorders. Increased serum TSH isan early and sensitive indicator of decreased thyroid reserve and overt primaryhypothyroidism. Decreased of TSH levels is an indicator of TSH-independenthyperthyroidism (Graves disease). The sensitivity of this ELISA test is 0.05IU/ml.