BT Labs Human Epithelial Cell Adhesion Molecule, EP-CAM Elisa Kit

Product Description

Intended Use

This Sandwich kit is for the accurate quantitative detection of Human Epithelial Cell Adhesion Molecule (also known as Ep-CAM) in serum, plasma, cell culture supernatants, Ascites, tissue homogenates or other biological fluids.

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human Ep-CAM antibody. EpCAM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Ep-CAM Antibody is added and binds to Ep-CAM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Ep-CAM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human Ep-CAM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.