Calbiotech AMH Elisa Kit

Product Description

PRINCIPLE OF THE TEST

The Calbiotech Inc., AMH ELISA is based on solid phase sandwich ELISA method. The samples

and conjugate reagent (anti-AMH biotin & HRP) are added to the wells coated with Streptavidin.

AMH in the patient's serum binds to the matched pair Abs, forming a sandwich complex and

simultaneously the complex is being immobilized on the plate through streptavidin-biotin

interactions. Unbound protein and HRP conjugate are washed off, through a washing step. Upon

addition of the substrate, the intensity of color is proportional to the concentration of AMH in the

samples. A standard curve is prepared by relating the color intensity to the concentration of

AMH.