Calbiotech HCG Elisa Kit

Product Description

SUMMARY AND EXPLANATION
Human Chorionic Gonadotropin (hCG) is a 40 kD glycoprotein hormone secreted by the placenta. hCGhas two subunits, alpha and beta. The alpha subunit is similar to the alpha subunit found in LH, FSH andTSH glycoprotein hormones. However, the beta subunit is specific and differs from hormone to hormone.The serum hCG rises in early pregnancy to concentrations of 50,000-150,000 mIU/ml between the 8th and12th weeks of gestation and decline to 20,000 mIU/ml by the 18th week where they remain for the durationof the pregnancy. The increased level of hCG in non-pregnant women or men suggest neoplasia. ThushCG measurement is useful for the recognition and monitoring of chorionic tumors and as a tumor markerfor other malignancies that produce hCG ectopically. These include testicular, pancreatic, andbronchogenic pulmonary cancers. 

PRINCIPLE OF THE TEST
The CBI hCG ELISA is an adapted solid phase sandwich ELISA method. The samples, biotin labeled antihCG and anti-hCG-HRP conjugate are added to the wells coated with Streptavidin. hCG in the patient™sserum binds to binds to the anti-hCG antibodies and forms a sandwich on the streptavidin coated plate.Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate,the intensity of color is proportional to the concentration of hCG in the samples. A standard curve isprepared relating color intensity to the concentration of hCG in the sample.

STORAGE AND STABILITY
1. Store the kit at 2 - 8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.