Calbiotech Prolactin Elisa Kit

Product Description

PRINCIPLE OF THE TEST
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, bindsto the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to theantibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added.The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the colorgenerated is proportional to the amount of IgG specific antibody in the sample.

SUMMARY AND EXPLANATION
Mycoplasma pneumoniae is a pathogen with spectrum of clinical presentations ranging from asymptomaticto pronounced pneumonia. Symptoms start from 6 to 32 days after exposure with headache, malaise,cough, sore throat and fever. The illness can last from a few days to a month or more. Detection by ELISAof M. pneumoniae IgM antibodies or demonstration of a significant increase of specific IgG antibodies isstrong evidence for recent infection in the appropriate clinical setting. Specific IgM antibodies typicallyincrease significantly 1 week after clinical onset and specific IgG levels rise in the second week. M.pneumoniae IgM can, however, persist for more than two years after infection, and therefore, detection ofspecific IgM does not accurately indicate the time of infection. Primary infection and reinfection may bedistinguished by the presence of elevated specific IgA and of specific IgM in primary infections and by thepresence of elevated specific IgA in the absence of specific IgM in reinfections. In general, the absence ofspecific IgM in serum collected 10-20 days after onset is strong evidence against primary pneumonia dueto M. pneumoniae.

STORAGE AND STABILITY1. Store the kit at 2-8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.