Calbiotech Thyroglobulin Ab (TG) Elisa Kit

About Calbiotech Thyroglobulin Ab (TG) Elisa Kit

SUMMARY AND EXPLANATION
Thyroglobulin is a water soluble glycoprotein that is involved in the storage and synthesis of thyroidhormones. The thyroid microsomal antigen has been shown to be the enzyme thyroid peroxidase (TPO).Antibodies to thyroglobulin and or microsomal antigen are present in most patients with goitrous thyroiditis(Hashimoto disease), atrophic thyroiditis and about 70-90% of Graves disease. Antibodies are also found inabout half of the patients with primary hypothyroidism and thyrotoxicosis, and 10-20% of patients withsimple goiters and thyroid tumors. There is also a relationship between thyroid antibodies and diabetesmellitus. Thyroid autoantibodies are present in about 6-7% of normals and their incidence increases withage. Classically, autoantibodies to thyroid antigens are detected by precipitation reactions,hemagglutination and by immunoflourescence. However the tests are subjective and lack high sensitivity.Enzyme-Linked Immunosorbent Assays (ELISAs) combine greater sensitivity, objective reading and ease ofuse. ELISAs have been developed and validated for detecting autoantibodies to thyroid antigens.

PRINCIPLE OF THE TEST
Diluted patient serum is added to wells coated with purified TG recombinant antigen. TG specific antibody,if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate isadded to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off andsubstrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. Theintensity of the color generated is proportional to the amount of TG specific antibody in the sample.