Calbiotech Mitochondrial (MA) Ab Elisa Kit

Product Description

SUMMARY AND EXPLANATION
Mitochondrial Antibodies (MA) are directed against the E2 subunit of the pyruvatedehydrogenase enzyme complex located at the inner mitochondrial membrane (PDC-E2), the E2subunit of the branched chain 2-oxo acid dehydrogenase complex (BCOADC-E2), the E2 subunitof the 2-oxo-glutarate dehydrogenase complex (OGDC-E2), protein X, and PDC-Ela and PDCE1. MA are found in 95% of patients with primary biliary cirrhosis (PBC). MA in low titers arecommon in chronic active hepatitis and their presence does not preclude response tocorticosteroids. MA disappear in about one month after orthotopic liver ransplantation (OLT) anddecrease with cyclosporine treatment which might be useful in PBC. MA are found in <1% ofapparently healthy Caucasoid adults. Approximately 3% of patients with PBC have scleroderma,usually of the CREST syndrome variety. In addition, MA reactive with the PDC-E2 complex arefound in some patients with CREST or diffuse scleroderma, sometimes in the absence of overtliver disease. Scleroderma typically precedes PBC in those patients with both diseases.

PRINCIPLE OF THE TEST
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, ifpresent, binds to the antigen. All unbound materials are washed away and the enzymeconjugate is added to bind to the antibody-antigen complex, if present. Excess enzymeconjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis ofthe substrate by the enzyme. The intensity of the color generated is proportional to the amountof IgG specific antibody in the sample.