Calbiotech Progesterone Elisa Kit

About Calbiotech Progesterone Elisa Kit

PRINCIPLE OF THE TEST
The Calbiotech, Inc progesterone is a solid phase competitive ELISA. The samples, working progesteroneenzyme (HRP) Conjugate and anti-progesterone-Biotin reagent are added to the wells coated withStreptavidin. Progesterone in the patient™s specimen competes with a progesterone HRP conjugate forbinding sites. Unbound Progesterone and Progesterone enzyme conjugate is washed off by washing buffer.Upon the addition of the TMB substrate, the intensity of color is inversely proportional to the concentrationof Progesterone in the samples. A standard curve is prepared relating color intensity to the concentration ofthe Progesterone.

SUMMARY AND EXPLANATION
Progesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. Cholesterolis transformed to Progesterone which is then converted via a combined dehydrogenase and isomerase toprogesterone. The principle production sites are the adrenals and ovaries and the placenta duringpregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as aglucuronide prior to excretion by the kidneys. Progesterone exhibits a wide variety of end organ effects. Theprimary role of progesterone is exhibited by the reproductive organs. In males, progesterone is a necessaryintermediate for the production of corticosteroids and androgens. In females, progesterone remainsrelatively constant throughout the follicular phase of the menstrual cycle. The concentration then increasesrapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hoursbefore the onset of menstruation. In pregnancy, placental progesterone production rises steadily to levelsof 10 to 20 times those of the luteal phase peak. Progesterone measurements are thus performed todetermine ovulation as well as to characterize luteal phase defects. Monitoring of progesterone therapy andearly stage pregnancy evaluations comprise the remaining uses of progesterone assays. Progesterone EIAkits are designed for the measurement of total progesterone in human serum or plasma. 

STORAGE AND STABILITY
1. Store the kit at 2 “ 8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.